A highly efficient in vivo plasmid editing tool based on CRISPR-Cas12a and phage λ Red recombineering
نویسندگان
چکیده
منابع مشابه
CRISPR-Cas12a-Assisted Recombineering in Bacteria
Clustered regularly interspaced short palindromic repeat (CRISPR)-Cas12a (Cpf1) has emerged as an effective genome editing tool in many organisms. Here, we developed and optimized a CRISPR-Cas12a-assisted recombineering system to facilitate genetic manipulation in bacteria. Using this system, point mutations, deletions, insertions, and gene replacements can be easily generated on the chromosome...
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Clustered regularly-interspaced short palindromic repeats (CRISPR) is a new and effective genetic editing tool. CRISPR was initially found in bacteria to protect it from virus invasions. In the first step, specific DNA strands of virus are identified by guide RNA that is composed of crRNA and tracrRNA. Then RNAse III is required for producing crRNA from pre-crRNA. In The second step, a crRNA:tr...
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ژورنال
عنوان ژورنال: Journal of Genetics and Genomics
سال: 2019
ISSN: 1673-8527
DOI: 10.1016/j.jgg.2019.07.006